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Freezing and Thawing Human Embryonic Stem Cells
Since James Thomson et al developed a technique in 1998 to isolate and grow hES in culture, freezing cells for later use and thawing and expanding cells from a frozen stock have become important procedures performed in routine hES cell culture. Since hES cells are very sensitive to the stresses of f...
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| Autor principal: | |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2009
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3152242/ https://ncbi.nlm.nih.gov/pubmed/20035272 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1555 |
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