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Purification, crystallization and preliminary X-ray analysis of glucokinase from Streptomyces griseus in complex with glucose

Glucokinase catalyzes the phosphorylation of glucose using ATP to yield glucose 6-­phosphate. SgGlkA is a bacterial group III glucokinase from Streptomyces griseus that seems to play a regulatory role in carbon catabolite repression in this organism. SgGlkA was expressed in Escherichia coli, purifie...

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Detalhes bibliográficos
Main Authors: Miyazono, Ken-ichi, Tabei, Nobumitsu, Marushima, Kazuya, Ohnishi, Yasuo, Horinouchi, Sueharu, Tanokura, Masaru
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3151127/
https://ncbi.nlm.nih.gov/pubmed/21821894
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111022275
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