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Differentiating Passive from Transporter-mediated Uptake by PepT1: A Comparison and Evaluation of 4 Methods

INTRODUCTION: To quantify transmembrane transport of dipeptides by PepT1, passive uptake (non-PepT1 mediated) must be subtracted from total (measured) uptake. Three methods have been described to estimate passive uptake: perform experiments at cold temperatures, inhibit target dipeptide uptake with...

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Bibliografiske detaljer
Main Authors: Scow, Jeffrey S., Madhavan, Srivats, Chaudhry, Rizwan M., Zheng, Ye, Duenes, Judith A., Sarr, Michael G.
Format: Artigo
Sprog:Inglês
Udgivet: 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3150389/
https://ncbi.nlm.nih.gov/pubmed/21529830
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jss.2011.02.018
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