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DNA Extraction from 0.22 μM Sterivex Filters and Cesium Chloride Density Gradient Centrifugation
This method is used to extract high molecular weight genomic DNA from planktonic biomass concentrated on 0.22 μM Sterivex filters that have been treated with storage/lysis buffer and archived at -80°C, and to purify this DNA using a cesium chloride density gradient. The protocol begins with two one-...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
MyJove Corporation
2009
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3150049/ https://ncbi.nlm.nih.gov/pubmed/19767717 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1352 |
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