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DNA Extraction from 0.22 μM Sterivex Filters and Cesium Chloride Density Gradient Centrifugation

This method is used to extract high molecular weight genomic DNA from planktonic biomass concentrated on 0.22 μM Sterivex filters that have been treated with storage/lysis buffer and archived at -80°C, and to purify this DNA using a cesium chloride density gradient. The protocol begins with two one-...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Päätekijät: Wright, Jody J., Lee, Sangwon, Zaikova, Elena, Walsh, David A., Hallam, Steven J.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: MyJove Corporation 2009
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC3150049/
https://ncbi.nlm.nih.gov/pubmed/19767717
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1352
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