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Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
In classical protein staining protocols using Coomassie Brilliant Blue (CBB), solutions with high contents of toxic and flammable organic solvents (Methanol, Ethanol or 2-Propanol) and acetic acid are used for fixation, staining and destaining of proteins in a gel after SDS-PAGE. To speed up the pro...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3149912/ https://ncbi.nlm.nih.gov/pubmed/19684570 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1350 |
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