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Reproducible gene targeting in recalcitrant Escherichia coli isolates

BACKGROUND: A number of allele replacement methods can be used to mutate bacterial genes. For instance, the Red recombinase system of phage Lambda has been used very efficiently to inactivate chromosomal genes in E. coli K-12, through recombination between regions of homology. However, this method d...

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Main Authors: Derous, Veerle, Deboeck, Francine, Hernalsteens, Jean-Pierre, De Greve, Henri
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2011
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3146853/
https://ncbi.nlm.nih.gov/pubmed/21696605
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-4-213
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