Pyrosequencing for Mini-Barcoding of Fresh and Old Museum Specimens

DNA barcoding is an effective approach for species identification and for discovery of new and/or cryptic species. Sanger sequencing technology is the method of choice for obtaining standard 650 bp cytochrome c oxidase subunit I (COI) barcodes. However, DNA degradation/fragmentation makes it difficu...

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Bibliographic Details
Main Authors: Shokralla, Shadi, Zhou, Xin, Janzen, Daniel H., Hallwachs, Winnie, Landry, Jean-François, Jacobus, Luke M., Hajibabaei, Mehrdad
Format: Artigo
Language:Inglês
Published: Public Library of Science 2011
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3144868/
https://ncbi.nlm.nih.gov/pubmed/21818256
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0021252
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