Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking

In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way...

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Bibliografiske detaljer
Main Authors: Annibale, Paolo, Vanni, Stefano, Scarselli, Marco, Rothlisberger, Ursula, Radenovic, Aleksandra
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2011
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3144238/
https://ncbi.nlm.nih.gov/pubmed/21818365
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0022678
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