Discrimination of GLUT4 vesicle trafficking from fusion using a temperature-sensitive Munc18c mutant
To examine the temporal relationship between pre- and post-docking events, we generated a Munc18c temperature-sensitive mutant (Munc18c/TS) by substitution of arginine 240 with a lysine residue. At the permissive temperature (23°C), overexpression of both the wild type (Munc18c/WT) and the R240K mut...
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| Publicado no: | EMBO J |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2000
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC313977/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10899111/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/19.14.3565 |
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