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Discrimination of GLUT4 vesicle trafficking from fusion using a temperature-sensitive Munc18c mutant

To examine the temporal relationship between pre- and post-docking events, we generated a Munc18c temperature-sensitive mutant (Munc18c/TS) by substitution of arginine 240 with a lysine residue. At the permissive temperature (23°C), overexpression of both the wild type (Munc18c/WT) and the R240K mut...

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Detalhes bibliográficos
Main Authors: Thurmond, Debbie C., Pessin, Jeffrey E.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2000
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC313977/
https://ncbi.nlm.nih.gov/pubmed/10899111
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/19.14.3565
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