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Discrimination of GLUT4 vesicle trafficking from fusion using a temperature-sensitive Munc18c mutant
To examine the temporal relationship between pre- and post-docking events, we generated a Munc18c temperature-sensitive mutant (Munc18c/TS) by substitution of arginine 240 with a lysine residue. At the permissive temperature (23°C), overexpression of both the wild type (Munc18c/WT) and the R240K mut...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
2000
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC313977/ https://ncbi.nlm.nih.gov/pubmed/10899111 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/19.14.3565 |
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