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Wildtype and Engineered Monomeric Triosephosphate Isomerase from Trypanosoma brucei: Partitioning of Reaction Intermediates in D(2)O and Activation by Phosphite Dianion()

Product yields for the reactions of (R)-glyceraldehyde 3-phosphate (GAP) in D(2)O at pD 7.9 catalyzed by wildtype triosephosphate isomerase from Trypanosoma brucei brucei (Tbb TIM) and a monomeric variant (monoTIM) of this wildtype enzyme were determined by (1)H NMR spectroscopy, and were compared w...

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Main Authors: Malabanan, M. Merced, Go, Maybelle K., Amyes, Tina L., Richard, John P.
格式: Artigo
語言:Inglês
出版: 2011
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3138527/
https://ncbi.nlm.nih.gov/pubmed/21553855
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi2005416
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