Factors to consider in using [U-C]palmitate for analysis of sphingolipid biosynthesis by tandem mass spectrometry

This study describes the use of a stable-isotope labeled precursor ([U-(13)C]palmitate) to analyze de novo sphingolipid biosynthesis by tandem mass spectrometry. It also describes factors to consider in interpreting the data, including the isotope's location ((13)C appears in three isotopomers...

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Detalhes bibliográficos
Main Authors: Haynes, Christopher A., Allegood, Jeremy C., Wang, Elaine W., Kelly, Samuel L., Sullards, M. Cameron, Merrill, Alfred H.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2011
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3137025/
https://ncbi.nlm.nih.gov/pubmed/21586681
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D015586
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