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Biocompatible artificial DNA linker that is read through by DNA polymerases and is functional in Escherichia coli

A triazole mimic of a DNA phosphodiester linkage has been produced by templated chemical ligation of oligonucleotides functionalized with 5′-azide and 3′-alkyne. The individual azide and alkyne oligonucleotides were synthesized by standard phosphoramidite methods and assembled using a straightforwar...

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Detalhes bibliográficos
Main Authors: El-Sagheer, Afaf H., Sanzone, A. Pia, Gao, Rachel, Tavassoli, Ali, Brown, Tom
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3136318/
https://ncbi.nlm.nih.gov/pubmed/21709264
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1101519108
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