A system for production and rapid purification of large amounts of the Shiga toxin/Shiga-like toxin I B subunit.

We have constructed a plasmid expression vector (pSBC32) that encodes the B subunit of Shiga toxin/Shiga-like toxin I under control of the inducible trc promoter. The encoded B subunit is transported to the periplasmic space, allowing single-step purification of milligram amounts of this protein fro...

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Detalhes bibliográficos
Publicado no:Infect Immun
Main Authors: Calderwood, S B, Acheson, D W, Goldberg, M B, Boyko, S A, Donohue-Rolfe, A
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1990
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC313598/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2201641/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.58.9.2977-2982.1990
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