A system for production and rapid purification of large amounts of the Shiga toxin/Shiga-like toxin I B subunit.

We have constructed a plasmid expression vector (pSBC32) that encodes the B subunit of Shiga toxin/Shiga-like toxin I under control of the inducible trc promoter. The encoded B subunit is transported to the periplasmic space, allowing single-step purification of milligram amounts of this protein fro...

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Detaylı Bibliyografya
Asıl Yazarlar: Calderwood, S B, Acheson, D W, Goldberg, M B, Boyko, S A, Donohue-Rolfe, A
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1990
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC313598/
https://ncbi.nlm.nih.gov/pubmed/2201641
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