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Purification of an 80,000-Mr glycylprolyl peptidase from Bacteroides gingivalis.

An enzyme from Bacteroides gingivalis SUNYAB A7A1-28 that hydrolyzes the synthetic peptide glycyl-L-proline 4-methoxy-beta-naphthylamide was purified 1,040-fold by urea extraction, gel filtration, ion-exchange chromatography, and fast protein liquid chromatography. The molecular weight of the enzyme...

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Bibliographic Details
Published in:Infect Immun
Main Authors: Barua, P K, Neiders, M E, Topolnycky, A, Zambon, J J, Birkedal-Hansen, H
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1989
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Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC313480/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2744859/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.57.8.2522-2528.1989
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