Significance of the Glutamate-139 Residue of the V-Type Na(+)-ATPase NtpK Subunit in Catalytic Turnover Linked with Salt Tolerance of Enterococcus hirae

A Glu139Asp mutant of the NtpK subunit (kE139D) of Enterococcus hirae vacuolar-type ATPase (V-ATPase) lost tolerance to sodium but not to lithium at pH 10. Purified kE139D V-ATPase retained relatively high specific activity and affinity for the lithium ion compared to the sodium ion. The kE139 resid...

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Autores principales: Kawano-Kawada, Miyuki, Takahashi, Hiroko, Igarashi, Kazuei, Murata, Takeshi, Yamato, Ichiro, Homma, Michio, Kakinuma, Yoshimi
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2011
Materias:
Enzymes and Proteins
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3133306/
https://ncbi.nlm.nih.gov/pubmed/21602356
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.01537-10
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