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Detection and Resolution of Cryptosporidium Species and Species Mixtures by Genus-Specific Nested PCR-Restriction Fragment Length Polymorphism Analysis, Direct Sequencing, and Cloning

Molecular methods incorporating nested PCR-restriction fragment length polymorphism (RFLP) analysis of the 18S rRNA gene of Cryptosporidium species were validated to assess performance based on limit of detection (LoD) and for detecting and resolving mixtures of species and genotypes within a single...

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Autors principals: Ruecker, Norma J., Hoffman, Rebecca M., Chalmers, Rachel M., Neumann, Norman F.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3131642/
https://ncbi.nlm.nih.gov/pubmed/21498746
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02706-10
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