An Optimized Protocol for DNA Extraction from Wheat Seeds and Loop-Mediated Isothermal Amplification (LAMP) to Detect Fusarium graminearum Contamination of Wheat Grain

A simple, rapid, and efficient method for isolating genomic DNA from germinated seeds of wheat that is free from polysaccharides and polyphenols is reported. DNA was extracted, treated with RNase, measured and tested for completeness using agarose gel electrophoresis. DNA purification from wheat gra...

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Bibliographic Details
Main Authors: Abd-Elsalam, Kamel, Bahkali, Ali, Moslem, Mohamed, Amin, Osama E., Niessen, Ludwig
Format: Artigo
Language:Inglês
Published: Molecular Diversity Preservation International (MDPI) 2011
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3131572/
https://ncbi.nlm.nih.gov/pubmed/21747688
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms12063459
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