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Mass spectrometry-based identification of protein kinase substrates utilizing engineered kinases and thiophosphate labeling
Protein kinases constitute a large enzyme family with key roles in cellular signal transduction. One way to elucidate the functions of protein kinases is to systematically identify their downstream targets. We present here a simple and effective method to identify direct protein kinase substrates in...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3131159/ https://ncbi.nlm.nih.gov/pubmed/21743840 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/9780470559277.ch100151 |
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