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Refolding of recombinant Pasteurella haemolytica A1 glycoprotease expressed in an Escherichia coli thioredoxin gene fusion system

Pasteurella haemolytica A1 secretes an O-sialoglycoprotein endopeptidase (EC. 3.4.24.57) (glycoprotease: Gcp) which is specific for O-linked sialoglycoproteins. When the cloned gene is expressed in Escherichia coli, the recombinant glycoprotease (rGcp) is secreted to the peripalsm where it is presen...

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Main Authors: Watt, Mary-Anne, Lo, Reggie Y. C., Mellors, Alan
格式: Artigo
語言:Inglês
出版: Cell Stress Society International 1997
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC312996/
https://ncbi.nlm.nih.gov/pubmed/9314606
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