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Characterization of U4 and U6 interactions with the 5′ splice site using a S. cerevisiae in vitro trans-splicing system
Spliceosome assembly has been characterized as the ordered association of the snRNP particles U1, U2, and U4/U6·U5 onto pre-mRNA. We have used an in vitro trans-splicing/cross-linking system in Saccharomyces cerevisiae nuclear extracts to examine the first step of this process, 5′ splice site recogn...
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| 主要な著者: | , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Cold Spring Harbor Laboratory Press
2001
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC312745/ https://ncbi.nlm.nih.gov/pubmed/11485990 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gad.895601 |
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