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Characterization of U4 and U6 interactions with the 5′ splice site using a S. cerevisiae in vitro trans-splicing system

Spliceosome assembly has been characterized as the ordered association of the snRNP particles U1, U2, and U4/U6·U5 onto pre-mRNA. We have used an in vitro trans-splicing/cross-linking system in Saccharomyces cerevisiae nuclear extracts to examine the first step of this process, 5′ splice site recogn...

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Bibliografiset tiedot
Päätekijät: Johnson, Tracy L., Abelson, John
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Cold Spring Harbor Laboratory Press 2001
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC312745/
https://ncbi.nlm.nih.gov/pubmed/11485990
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gad.895601
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