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Methylation by a mutant T2 DNA [N(6)-adenine] methyltransferase expands the usage of RecA-assisted endonuclease (RARE) cleavage

Properties of a mutant bacteriophage T2 DNA [N(6)-adenine] methyltransferase (T2 Dam MTase) have been investigated for its potential utilization in RecA-assisted restriction endonuclease (RARE) cleavage. Steady-state kinetic analyses with oligonucleotide duplexes revealed that, compared to wild-type...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Minko, Irina, Hattman, Stanley, Lloyd, R. Stephen, Kossykh, Valeri
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2001
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC31273/
https://ncbi.nlm.nih.gov/pubmed/11266550
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