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A novel fixative for immunofluorescence staining of CD133-positive glioblastoma stem cells

Isolation of glioblastoma stem cells requires incubation of tumor cells in a neural stem cell media. Neurospheres containing these glioblastoma stem cells are formed after approximately a five-day period. These cells can then be analyzed for the presence of stem cell markers. Immunofluorescence stai...

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Detalhes bibliográficos
Main Authors: Sherman, Jonathan H., Redpath, Gerard T., Redick, Jan A., Purow, Benjamin W., Laws, Edward R., Jane, John A., Shaffrey, Mark E., Hussaini, Isa M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3126626/
https://ncbi.nlm.nih.gov/pubmed/21402102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jneumeth.2011.03.003
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