DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments.

The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was tested on single-stranded templates,...

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Main Authors: Lee, L G, Connell, C R, Woo, S L, Cheng, R D, McArdle, B F, Fuller, C W, Halloran, N D, Wilson, R K
Format: Artigo
Jezik:Inglês
Izdano: 1992
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC312381/
https://ncbi.nlm.nih.gov/pubmed/1598205
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