Cloning, expression, and characterization of TonB2 from Actinobacillus pleuropneumoniae and potential use as an antigenic vaccine candidate and diagnostic marker

In this study the tonB2 gene was cloned from Actinobacillus pleuropneumoniae JL01 (serovar 1) and expressed as a glutathione-S-transferase (GST) fusion protein in Escherichia coli BL21(DE3). The GST fusion protein was recognized by antibodies in serum positive for A. pleuropneumoniae by Western blot...

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Bibliographic Details
Main Authors: Liu, Jinlin, Chen, Yan, Yuan, Fangyan, Hu, Linlin, Bei, Weicheng, Chen, Huanchun
Format: Artigo
Language:Inglês
Published: Canadian Veterinary Medical Association 2011
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3122975/
https://ncbi.nlm.nih.gov/pubmed/22210994
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