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Comparison of Real-Time PCR Assays for Detection of Pathogenic Leptospira spp. in Blood and Identification of Variations in Target Sequences

Leptospirosis is considered an underdiagnosed disease. Although several PCR-based methods are currently in use, there is little information on their comparability. In this study, four quantitative real-time PCR (qPCR) assays (SYBR green and TaqMan chemistries) targeting the secY, lfb1, and lipL32 ge...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Bourhy, Pascale, Bremont, Sylvie, Zinini, Farida, Giry, Claude, Picardeau, Mathieu
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Microbiology 2011
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3122738/
https://ncbi.nlm.nih.gov/pubmed/21471336
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02452-10
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