Simultaneous Detection of Antibodies to Mouse Hepatitis Virus Recombinant Structural Proteins by a Microsphere-Based Multiplex Fluorescence Immunoassay

We describe a new microsphere-based multiplex fluorescent immunoassay (MFI) using recombinant mouse hepatitis virus (MHV) proteins to detect antibodies to coronaviruses in mouse and rat sera. All the recombinant proteins, including nucleocapsid (N) and 3 subunits of spike protein, S1, S2, and Smid,...

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Bibliographic Details
Main Authors: Kunita, Satoshi, Kato, Kanako, Ishida, Miyuki, Hagiwara, Kozue, Kameda, Shuko, Ishida, Tomoko, Takakura, Akira, Goto, Kazuo, Sugiyama, Fumihiro, Yagami, Ken-ichi
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2011
Subjects:
Veterinary Immunology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3122517/
https://ncbi.nlm.nih.gov/pubmed/21430123
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CVI.00467-10
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