The Utility of High-Resolution Melting Analysis of SNP Nucleated PCR Amplicons—An MLST Based Staphylococcus aureus Typing Scheme

High resolution melting (HRM) analysis is gaining prominence as a method for discriminating DNA sequence variants. Its advantage is that it is performed in a real-time PCR device, and the PCR amplification and HRM analysis are closed tube, and effectively single step. We have developed an HRM-based...

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Bibliografiske detaljer
Main Authors: Lilliebridge, Rachael A., Tong, Steven Y.C., Giffard, Philip M., Holt, Deborah C.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2011
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3120814/
https://ncbi.nlm.nih.gov/pubmed/21731606
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0019749
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