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A densely overlapping gene fragmentation approach improves yeast two-hybrid screens for Plasmodium falciparum proteins

Use of the yeast two-hybrid assay to study Plasmodium falciparum protein-protein interactions is limited by poor expression of P. falciparum genes in yeast and lack of easily implemented assays to confirm the results. We report here two methods to create gene fragments – random fragmentation by part...

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Detalhes bibliográficos
Main Authors: Brown, Hakeenah F., Wang, Ling, Khadka, Sudip, Fields, Stanley, LaCount, Douglas J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3120127/
https://ncbi.nlm.nih.gov/pubmed/21530591
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molbiopara.2011.04.005
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