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A densely overlapping gene fragmentation approach improves yeast two-hybrid screens for Plasmodium falciparum proteins

Use of the yeast two-hybrid assay to study Plasmodium falciparum protein-protein interactions is limited by poor expression of P. falciparum genes in yeast and lack of easily implemented assays to confirm the results. We report here two methods to create gene fragments – random fragmentation by part...

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Bibliografski detalji
Glavni autori: Brown, Hakeenah F., Wang, Ling, Khadka, Sudip, Fields, Stanley, LaCount, Douglas J.
Format: Artigo
Jezik:Inglês
Izdano: 2011
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3120127/
https://ncbi.nlm.nih.gov/pubmed/21530591
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molbiopara.2011.04.005
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