Reversed-phase chromatography with multiple fraction concatenation strategy for proteome profiling of human MCF10A cells

In this study, we evaluated a concatenated low pH (pH 3) and high pH (pH 10) reversed-phase liquid chromatography strategy as a first dimension for two-dimensional liquid chromatography tandem mass spectrometry (“shotgun”) proteomic analysis of trypsin-digested human MCF10A cell sample. Compared wit...

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Detalhes bibliográficos
Main Authors: Wang, Yuexi, Yang, Feng, Gritsenko, Marina A., Wang, Yingchun, Clauss, Therese, Liu, Tao, Shen, Yufeng, Monroe, Matthew E., Lopez-Ferrer, Daniel, Reno, Theresa, Moore, Ronald J., Klemke, Richard L., Camp, David G., Smith, Richard D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3120047/
https://ncbi.nlm.nih.gov/pubmed/21500348
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.201000722
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