Synthesis of isotope labeled oligonucleotides and their use in an NMR study of a protein-DNA complex.

The synthesis of an oligonucleotide labeled with 13C at the thymine methyl and 15N at the exocyclic amino groups of the cytosines is described. 13CH3I and 15NH4OH were used as sources of the labels. The labeled oligonucleotide was characterized by several NMR techniques. The duplex possesses a label...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Kellenbach, E R, Remerowski, M L, Eib, D, Boelens, R, van der Marel, G A, van den Elst, H, van Boom, J H, Kaptein, R
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1992
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC312000/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1542561/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.4.653
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