Deletion and rearrangement of plasmid DNA during transformation of Escherichia coli with linear plasmid molecules.
When E. coli was transformed with linearized pBR322 DNA, many transformants contained recircularized plasmids bearing deletions and other rearrangements. Most aberrant molecules were less than monomeric length and had lost the restriction site used for linearization, with the deleted region extendin...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1986
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| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311919/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3024124/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.22.8905 |
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