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In vitro mutagenesis of the putative replicase genes of tobacco mosaic virus.
We have established an in vitro transcription system to produce infectious tobacco mosaic virus (TMV) RNA from a cloned cDNA copy. Using this system, several TMV mutants were transcribed in vitro from cDNA clones mutagenized at or near the leaky amber termination codon of the 130K protein gene, and...
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| Hauptverfasser: | , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1986
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC311860/ https://ncbi.nlm.nih.gov/pubmed/3786131 |
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