Single-Shot Optical Sectioning Using Two-Color Probes in HiLo Fluorescence Microscopy

We describe a wide-field fluorescence microscope setup which combines HiLo microscopy technique with the use of a two-color fluorescent probe. It allows one-shot fluorescence optical sectioning of thick biological moving sample which is illuminated simultaneously with a flat and a structured pattern...

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Bibliografiske detaljer
Main Authors: Muro, Eleonora, Vermeulen, Pierre, Ioannou, Andriani, Skourides, Paris, Dubertret, Benoit, Fragola, Alexandra, Loriette, Vincent
Format: Artigo
Sprog:Inglês
Udgivet: The Biophysical Society 2011
Fag:
Spectroscopy, Imaging, and Other Techniques
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3117152/
https://ncbi.nlm.nih.gov/pubmed/21641327
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2011.03.047
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