Efficient isolation of the linear DNA killer plasmid of Kluyveromyces lactis: evidence for location and expression in the cytoplasm and characterization of their terminally bound proteins.

Differential centrifugation of an osmotic lysate of K. lactis protoplasts showed that the linear DNA killer plasmids of K. lactis, pGKL1 and pGKL2, are almost exclusively present in the cytoplasmic fraction. This fractionation procedure allows the rapid isolation of large amounts of plasmid DNA with...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Stam, J C, Kwakman, J, Meijer, M, Stuitje, A R
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 1986
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC311705/
https://ncbi.nlm.nih.gov/pubmed/3763395
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