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Interaction of the tight-binding I12-X86 lac repressor with non operator DNA: salt dependence of complex formation.

The interaction of the wild-type lac repressor and its tight binding double mutant I12-X86 with a non operator-210 base pair-DNA fragment has been investigated using the nitrocellulose filter binding assay. While the affinity of the double mutant for this non specific DNA is increased as compared to...

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Publicat a:Nucleic Acids Res
Autors principals: Grebert, P, Maurizot, J C
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1986
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311668/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3529038/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.16.6613
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