The use of single-stranded DNA and RNase H to promote quantitative 'hybrid arrest of translation' of mRNA/DNA hybrids in reticulocyte lysate cell-free translations.

Single-stranded cDNA clones complementary to the 5' end of TMV RNA have been used to explore the conditions necessary for efficient 'hybrid arrest of translation' in the reticulocyte lysate. It is shown that incubations of 20 minutes at 60 degrees in 0.1 M KCl are sufficient to give a...

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Main Authors: Minshull, J, Hunt, T
Format: Artigo
Jezik:Inglês
Izdano: 1986
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC311656/
https://ncbi.nlm.nih.gov/pubmed/3018671
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