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Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions

Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was developed to...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Phan, Hoang Trong, Conrad, Udo
Format: Artigo
Sprache:Inglês
Veröffentlicht: Molecular Diversity Preservation International (MDPI) 2011
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3116158/
https://ncbi.nlm.nih.gov/pubmed/21686152
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms12052808
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