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Isolation and computer-aided characterization of MmeI, a type II restriction endonuclease from Methylophilus methylotrophus.

A Type II restriction endonuclease, MmeI, has been purified from the obligate methylotroph, Methylophilus methylotrophus. The enzyme was shown to have the non-palindromic recognition sequence 5'-T C C Pu A C (N)20-3', 3'-A G G Py T G (N)18-5' and to cleave (as indicated) on the 3' side, generating a...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Principais autores: Boyd, A C, Charles, I G, Keyte, J W, Brammar, W J
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1986
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311539/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3016643/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.13.5255
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