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The DNA-dependent RNA-polymerase of Thermotoga maritima; characterisation of the enzyme and the DNA-sequence of the genes for the large subunits.

An improved purification procedure for Thermotoga maritima RNA-polymerase holoenzyme was developed. The enzyme is highly active with poly dAT or T7 phage DNA as template. DNA gyrase was found to be a side product of this RNA-polymerase purification. The genes for the large subunits beta and beta' of...

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Udgivet i:Nucleic Acids Res
Principais autores: Palm, P, Schleper, C, Arnold-Ammer, I, Holz, I, Meier, T, Lottspeich, F, Zillig, W
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1993
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311404/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8177738/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.21.4904
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