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Enhanced transfection of cell lines from Atlantic salmon through nucoleofection and antibiotic selection
BACKGROUND: Cell lines from Atlantic salmon kidney have made it possible to culture and study infectious salmon anemia virus (ISAV), an aquatic orthomyxovirus affecting farmed Atlantic salmon. However, transfection of these cells using calcium phosphate precipitation or lipid-based reagents shows ve...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3113957/ https://ncbi.nlm.nih.gov/pubmed/21548922 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-4-136 |
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