A two-step species-specific 16S rRNA PCR assay for the detection of Taylorella equigenitalis in horses

A two-step PCR assay was developed for the molecular detection of Taylorella equigenitalis, a Gram-negative genital bacterial pathogen in horses. Two specific oligonucleotide primers (TE16SrRNABCHf [25mer] and TE16SrRNABCHr [29mer]) were designed from multiple alignments of the 16S rRNA gene loci of...

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Detalhes bibliográficos
Main Authors: Buckley, Thomas C, Millar, B Cherie, Egan, Claire L, Gibson, Paula, Cosgrove, Hazel, Stanbridge, Siobhan, Matsuda, Motoo, Moore, John E
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2005
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3113911/
https://ncbi.nlm.nih.gov/pubmed/21851668
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/2046-0481-58-3-146
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