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Electrostatic surface plasmon resonance: Direct electric field-induced hybridization and denaturation in monolayer nucleic acid films and label-free discrimination of base mismatches

We demonstrate that in situ optical surface plasmon resonance spectroscopy can be used to monitor hybridization kinetics for unlabeled DNA in tethered monolayer nucleic acid films on gold in the presence of an applied electrostatic field. The dc field can enhance or retard hybridization and can also...

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Bibliografski detalji
Glavni autori: Heaton, Richard J., Peterson, Alexander W., Georgiadis, Rosina M.
Format: Artigo
Jezik:Inglês
Izdano: The National Academy of Sciences 2001
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC31115/
https://ncbi.nlm.nih.gov/pubmed/11259682
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.071623998
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