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Fluorescence Polarization in Homogeneous Nucleic Acid Analysis II: 5′-Nuclease Assay

When the temperature and viscosity of the solvent is held constant, the degree of fluorescence polarization (FP) detected when a fluorescent dye is excited by plane polarized light depends mostly on the molecular weight of the dye molecule. By monitoring the FP of a fluorescent dye molecule, one can...

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Udgivet i:Genome Res
Principais autores: Latif, Sherif, Bauer-Sardina, Irma, Ranade, Kostubh, Livak, Kenneth J., Kwok, Pui-Yan
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2001
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Methods
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311069/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11230167/
https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gr.156601
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