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Relevance of BAC transgene copy number in mice: transgene copy number variation across multiple transgenic lines and correlations with transgene integrity and expression

Bacterial artificial chromosomes (BACs) are excellent tools for manipulating large DNA fragments and, as a result, are increasingly utilized to engineer transgenic mice by pronuclear injection. The demand for BAC transgenic mice underscores the need for careful inspection of BAC integrity and fideli...

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Detalhes bibliográficos
Main Authors: Chandler, Kelly J., Chandler, Ronald L., Broeckelmann, Eva M., Hou, Yue, Southard-Smith, E. Michelle, Mortlock, Douglas P.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3110064/
https://ncbi.nlm.nih.gov/pubmed/17882484
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00335-007-9056-y
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