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Calibrating excitation light fluxes for quantitative light microscopy in cell biology
Power output of light bulbs changes over time and the total energy delivered will depend on the optical beam path of the microscope, filter sets and objectives used, thus making comparison between experiments performed on different microscopes complicated. Using a thermocoupled power meter, it is po...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3109976/ https://ncbi.nlm.nih.gov/pubmed/18974739 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2008.180 |
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