Rapid Detection of Deletion, Insertion, and Substitution Mutations via Heteroduplex Analysis Using Capillary- and Microchip-Based Electrophoresis

In this report, we explore the potential of capillary and microchip electrophoresis for heteroduplex analysis– (HDA) based mutation detection. Fluorescent dye-labeled primers (6-FAM-tagged) were used to amplify the DNA fragments ranging from 130 to 400 bp. The effects of DNA fragment length, matrix...

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Detaylı Bibliyografya
Asıl Yazarlar: Tian, Huijun, Brody, Lawrence C., Landers, James P.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Cold Spring Harbor Laboratory Press 2000
Konular:
Methods
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC310899/
https://ncbi.nlm.nih.gov/pubmed/10984458
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