In Vivo Analysis of Proteomes and Interactomes Using Parallel Affinity Capture (iPAC) Coupled to Mass Spectrometry

Affinity purification coupled to mass spectrometry provides a reliable method for identifying proteins and their binding partners. In this study we have used Drosophila melanogaster proteins triple tagged with Flag, Strep II, and Yellow fluorescent protein in vivo within affinity pull-down experimen...

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Bibliografiska uppgifter
Huvudupphovsmän: Rees, Johanna S., Lowe, Nick, Armean, Irina M., Roote, John, Johnson, Glynnis, Drummond, Emma, Spriggs, Helen, Ryder, Edward, Russell, Steven, Johnston, Daniel St, Lilley, Kathryn S.
Materialtyp: Artigo
Språk:Inglês
Publicerad: The American Society for Biochemistry and Molecular Biology 2011
Ämnen:
Technological Innovation and Resources
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3108830/
https://ncbi.nlm.nih.gov/pubmed/21447707
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M110.002386
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