In Vivo Analysis of Proteomes and Interactomes Using Parallel Affinity Capture (iPAC) Coupled to Mass Spectrometry

Affinity purification coupled to mass spectrometry provides a reliable method for identifying proteins and their binding partners. In this study we have used Drosophila melanogaster proteins triple tagged with Flag, Strep II, and Yellow fluorescent protein in vivo within affinity pull-down experimen...

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Main Authors: Rees, Johanna S., Lowe, Nick, Armean, Irina M., Roote, John, Johnson, Glynnis, Drummond, Emma, Spriggs, Helen, Ryder, Edward, Russell, Steven, Johnston, Daniel St, Lilley, Kathryn S.
Format: Artigo
Jezik:Inglês
Izdano: The American Society for Biochemistry and Molecular Biology 2011
Teme:
Technological Innovation and Resources
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3108830/
https://ncbi.nlm.nih.gov/pubmed/21447707
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M110.002386
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