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Protease inhibitors as possible pitfalls in proteomic analyses of complex biological samples

Sample preparation, especially protein and peptide fractionation prior to identification by mass spectrometry (MS) are typically applied to reduce sample complexity. The second key element in this process is proteolytic digestion that is performed mostly by trypsin. Optimization of this step is an i...

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Bibliographische Detailangaben
Hauptverfasser: Clifton, James, Huang, Feilei, Rucevic, Marijana, Cao, Lulu, Hixson, Douglas, Josic, Djuro
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2011
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3107918/
https://ncbi.nlm.nih.gov/pubmed/21333769
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jprot.2011.02.010
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