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Improved DNA Sequencing Accuracy and Detection of Heterozygous Alleles Using Manganese Citrate and Different Fluorescent Dye Terminators

The use of dideoxynucleotide triphosphates labeled with different fluorescent dyes (dye terminators) is the most versatile method for automated DNA sequencing. However, variation in peak heights reduces base-calling accuracy and limits heterozygous allele detection, favoring use of dye-labeled prime...

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Bibliografiske detaljer
Main Authors: Korch, Christopher, Drabkin, Harry
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 1999
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC310770/
https://ncbi.nlm.nih.gov/pubmed/10400927
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